pFS461
(Plasmid #89064)

• Purpose: leu1 integration plasmid with adh1pr:Z3EV
• Depositing Lab: Nick Rhind
• Publication: Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1002/yea.3235. Epub 2017 Jun 8.

Backbone

• Vector backbone: pFS181 (pSS1)
• Backbone manufacturer: Rhind Lab
• Backbone size w/o insert (bp): 5285
• Total vector size (bp): 6683
• Vector type: leu1 integrating vector
• Selectable markers: S. pombe Leu1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Z3EV artificial transcription factor
• Species: Synthetic
• Insert Size (bp): 1398
• Promoter: adh1

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: actctagaggatctcat
• 3′ sequencing primer: GTCATAGCTGTTTCCTG

Resource Information

• Supplemental Documents:
  • pFS461.gb
• A portion of this plasmid was derived from a plasmid made by: McIsaac, R.S. et al., 2011. Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae. Molecular biology of the cell, 22(22), pp.4447–59. McIsaac, R.S. et al., 2014. Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae. Nucleic Acids Research, 42(6), pp.1–8.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry