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Addgene

pPS811
(Plasmid #8904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPS808
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPL3
  • Alt name
    pGAL-GFP-NPL3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1500
  • Entrez Gene
    NPL3 (a.k.a. YDR432W, MTR13, MTS1, NAB1, NOP3)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GAL1 promoter, 2 micron plasmid. 1.5kb BamHI fragment from pPS425. See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS811 was a gift from Pamela Silver (Addgene plasmid # 8904 ; http://n2t.net/addgene:8904 ; RRID:Addgene_8904)

  • For your References section:

    A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Lee MS, Henry M, Silver PA. Genes Dev 1996 May 15;10(10):1233-46. 10.1101/gad.10.10.1233 PubMed 8675010
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