Skip to main content
Addgene

tet operator
(Plasmid #8901)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8901 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tet operator
  • Species
    E. Coli
  • Insert Size (bp)
    282

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the tet operator can be released from this plasmid using a XhoI digest.

The construct is composed of the tet operator sequences followed by a CMV minimal promoter. The insert was digested with XhoI and EcoRI, klenow filled, and cloned into the SmaI site of pBluescript KS. See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tet operator was a gift from Lynda Chin (Addgene plasmid # 8901 ; http://n2t.net/addgene:8901 ; RRID:Addgene_8901)

  • For your References section:

    Essential role for oncogenic Ras in tumour maintenance. Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, Shen Q, O'Hagan R, Pantginis J, Zhou H, Horner JW, Cordon-Cardo C, Yancopoulos GD, DePinho RA. Nature. 1999 Jul 29. 400(6743):468-72. 10.1038/22788 PubMed 10440378
Related items:
Commonly requested with: