tet H-ras val12
(Plasmid #8900)

• Depositing Lab: Lynda Chin
• Publication: Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

Backbone

• Vector backbone: pBluescript KS
• Backbone size w/o insert (bp): 3350
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ras val12
• Alt name: ras
• Alt name: H-ras
• Species: H. sapiens (human)
• Insert Size (bp): 2700
• Mutation: G12V
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Tag / Fusion Protein:
  • Tet-op promoter (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RasV12 was isolated from a fragment of pRIPI-cH-Ras, a gift of Shimon Efrat.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The construct is composed of the following elements: 1) 350bp Tet-operators with CMV minimal promoter, 2) 1.8kb rasVal12, 3) SV40 splice and polyadenylation sequences. The entire 3050bp insert containing rasV12 and regulatory elements can be released with NotI. See author's map for more information.