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Addgene

Gal4 PGC1 alpha 1-550
(Plasmid #8894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMX Gal4
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1 alpha
  • Alt name
    PPAR gamma coactivator 1
  • Alt name
    PGC1a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1650
  • Mutation
    amino acids 1-550 of PGC-1 alpha
  • GenBank ID
    NM_008904
  • Entrez Gene
    Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
  • Tag / Fusion Protein
    • Gal4 DBD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer GAGTAGTAACAAAGGTCAA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PGC1(1-550) was cloned downstream of the Gal4 DNA binding domain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal4 PGC1 alpha 1-550 was a gift from Bruce Spiegelman (Addgene plasmid # 8894 ; http://n2t.net/addgene:8894 ; RRID:Addgene_8894)

  • For your References section:

    Activation of PPARgamma coactivator-1 through transcription factor docking. Puigserver P, Adelmant G, Wu Z, Fan M, Xu J, O'Malley B, Spiegelman BM. Science 1999 Nov 12;286(5443):1368-71. 10.1126/science.286.5443.1368 PubMed 10558993
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