7xNLS-pMJ915v2-sfGFP
(Plasmid
#88922)
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PurposeFor expression of modified SpyCas9 in e.coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 88922 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMJ915
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCas9
Gene/Insert 2
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Gene/Insert nameT7
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
7xNLS-pMJ915v2-sfGFP was a gift from Jennifer Doudna (Addgene plasmid # 88922 ; http://n2t.net/addgene:88922 ; RRID:Addgene_88922) -
For your References section:
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes. Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806. 10.1038/nbt.3806 PubMed 28191903