pLZ12Km2-P23R:TA:fflucRT
(Plasmid #88901)

• Purpose: Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression
• Depositing Lab: Thomas Proft
• Publication: Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 10.1007/s00253-013-5200-7. Epub 2013 Sep 6.

Backbone

• Vector backbone: pLZ12Km2
• Backbone manufacturer: Nobuhiko Okada (PMID: 9846743)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: toxin-antitoxin + Red-shifted firefly luciferase
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The P23 promoter sequence was cloned from pOri23 (Philippe Moreillon; PMID: 10816506). The toxin-antitoxin cassette was cloned from pBT286 (Urszula Zielenkiewicz; PMID: 16109951).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784