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Purpose(Empty Backbone) Carries phiC31 integrase attB site flanked by cHS4 insulators.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 88896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
- Promoter CAG
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsDepositor recommends DH10B, XL1 blue or NEB's DH10-beta for growth. DH5alpha should not be used as it does not yield good transformation results
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC-HBH was a gift from Michele Calos (Addgene plasmid # 88896 ; http://n2t.net/addgene:88896 ; RRID:Addgene_88896) -
For your References section:
Recombinase-mediated reprogramming and dystrophin gene addition in mdx mouse induced pluripotent stem cells. Zhao C, Farruggio AP, Bjornson CR, Chavez CL, Geisinger JM, Neal TL, Karow M, Calos MP. PLoS One. 2014 Apr 29;9(4):e96279. doi: 10.1371/journal.pone.0096279. eCollection 2014. 10.1371/journal.pone.0096279 PubMed 24781921