pTGD
(Plasmid
#88894)
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Purpose(Empty Backbone) Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 88894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTrcHis2B
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byProf. Gregory Stephanopoulos of MIT.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTGD was a gift from Keith Tyo (Addgene plasmid # 88894 ; http://n2t.net/addgene:88894 ; RRID:Addgene_88894) -
For your References section:
Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering. Love AM, Biggs BW, Tyo KEJ, Ajikumar PK. Methods Mol Biol. 2019;1927:37-45. doi: 10.1007/978-1-4939-9142-6_4. 10.1007/978-1-4939-9142-6_4 PubMed 30788784
Map uploaded by the depositor.
