p28-2iDP-puro
(Plasmid #88893)

• Purpose: Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.
• Depositing Lab: Michele Calos
• Publication: Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/biot.201600477.

Backbone

• Vector backbone: p28
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Growth instructions: Depositor recommends DH10B, XL1 blue or NEB's DH10-beta for growth. DH5alpha should not be used as it does not yield good transformation results
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Dual-intron DMD platform
• Alt name: 2iDP
• Species: H. sapiens (human)
• Insert Size (bp): 1236
• Mutation: Truncated version of the dystrophin protein (transcript variant Dp427m) that consists of residues 1-199 and 3554-3685 connected by a 13-amino acid linker.

Gene/Insert 2

• Gene/Insert name: luciferase
• Alt name: luc
• Species: Firefly
• Insert Size (bp): 1647

Gene/Insert 3

• Gene/Insert name: puromycin resistance enzyme
• Alt name: puroR
• Insert Size (bp): 594
• Mutation: codon optimized

Gene/Insert 4

• Gene/Insert name: mCherry
• Alt name: red
• Insert Size (bp): 705

Resource Information

• Supplemental Documents:
  • p28-2iDP-puro.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry