pAPM sh2 DNMT1
(Plasmid #88885)

• Purpose: Lentiviral vector expressing shRNA against murine DNMT1
• Depositing Lab: Silvia Monticelli
• Publication: Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 10.1073/pnas.1616420114.

Backbone

• Vector backbone: pAPM
• Backbone size w/o insert (bp): 7505
• Total vector size (bp): 7617
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shRNA no.2 DNMT1
• gRNA/shRNA sequence: AAGGCTCGAGAAGGTATATTGCTGTTGACAGTGAGCGCACAAACGCTCTCATCGACAAGT GTGACGTCGAAGACTCCTGACTTGTCGATGAGAGCGTTTGTATGCCTACTGCCTCGGAAT TCAAGGGGCT
• Species: M. musculus (mouse)
• Promoter: SFFV (spleen focus forming virus)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GGTTTATTACAGGGACAGCAGAG
• 3′ sequencing primer: CCACCGGTATCGATACGCGTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry