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pcDHa_mDDX5_FL_K144N
(Plasmid #88870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 88870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDHa
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX5
  • Alt name
    p68
  • Alt name
    DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1848
  • Mutation
    K144N
  • GenBank ID
    13207
  • Entrez Gene
    Ddx5 (a.k.a. 2600009A06Rik, G17P1, HUMP68, Hlr1, p68)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDHa_mDDX5_FL_K144N was a gift from Dan Littman (Addgene plasmid # 88870 ; http://n2t.net/addgene:88870 ; RRID:Addgene_88870)

  • For your References section:

    DDX5 and its associated lncRNA Rmrp modulate TH17 cell effector functions. Huang W, Thomas B, Flynn RA, Gavzy SJ, Wu L, Kim SV, Hall JA, Miraldi ER, Ng CP, Rigo F, Meadows S, Montoya NR, Herrera NG, Domingos AI, Rastinejad F, Myers RM, Fuller-Pace FV, Bonneau R, Chang HY, Acuto O, Littman DR. Nature. 2015 Dec 24;528(7583):517-22. doi: 10.1038/nature16193. Epub 2015 Dec 16. 10.1038/nature16193 PubMed 26675721
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