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Addgene

PGC-1 alpha promoter 2kb luciferase Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: KpnI + XhoI.

We expected the following band sizes for this digest: 4.8kb and 2.6kb. We see the expected pattern. The upper band running at ~8kb is uncut plasmid.