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pRK5-myc-PAT1
(Plasmid #88856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 88856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-myc
  • Backbone size w/o insert (bp) 4718
  • Total vector size (bp) 6149
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SLC36A1
  • Alt name
    PAT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1431
  • GenBank ID
    Gene ID: 206358 NM_078483.3
  • Entrez Gene
    SLC36A1 (a.k.a. Dct1, LYAAT1, PAT1, TRAMD3)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer SP6 : ATT TAG GTG ACA CTA TAG
  • 3′ sequencing primer RK5-3': TGT AAC CAT TAT AAG CTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-myc-PAT1 was a gift from David Sabatini (Addgene plasmid # 88856 ; http://n2t.net/addgene:88856 ; RRID:Addgene_88856)

  • For your References section:

    mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase. Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM. Science. 2011 Nov 4;334(6056):678-83. 10.1126/science.1207056 PubMed 22053050
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