Skip to main content
Addgene

pSV Sport SREBP-1c dom neg
(Plasmid #8885)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSV-Sport
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3160
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SREBP-1c
  • Alt name
    ADD1
  • Alt name
    SREBP
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • Mutation
    Y305A amino acid substitution in the active version of SREBP-1c (K312R). Creates a dominant negative version of SREBP-1c.
  • Entrez Gene
    Srebf1 (a.k.a. ADD-1, ADD1, SREBP-1, SREBP-1c, Srebp1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SREBP-1c cloned from a lambda gt11 rat adipocyte cDNA expression library.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV Sport SREBP-1c dom neg was a gift from Bruce Spiegelman (Addgene plasmid # 8885 ; http://n2t.net/addgene:8885 ; RRID:Addgene_8885)

  • For your References section:

    ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Kim JB, Spiegelman BM. Genes Dev. 1996 May 1. 10(9):1096-107. 10.1101/gad.10.9.1096 PubMed 8654925
Related items:
Commonly requested with: