IMBB2.4-pUC57-mini
(Plasmid
#88845)
-
Purpose(Empty Backbone) plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 88845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepUC57-mini
-
Backbone manufacturerGenScript
- Backbone size (bp) 2146
-
Modifications to backbonepolylinker/multiple cloning site
-
Vector typeSynthetic Biology ; cloning
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
Resource Information
-
A portion of this plasmid was derived from a plasmid made bypolylinker synthesized and cloned by GenScript
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
IMBB2.4-pUC57-mini was a gift from Ichiro Matsumura (Addgene plasmid # 88845 ; http://n2t.net/addgene:88845 ; RRID:Addgene_88845) -
For your References section:
Semi-automated Tip Snip cloning of restriction fragments into and out of plasmid polylinkers. Matsumura I. Biotechniques. 2017 Mar 1;62(3):99-106. doi: 10.2144/000114522. 10.2144/000114522 PubMed 28298176