Skip to main content
Addgene

RHPMP190 (pLifeAct tagRFP-T)
(Plasmid #88842)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 88842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    mEos4b-Lifeact Plasmid #54813
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LifeAct
  • Promoter CMV
  • Tag / Fusion Protein
    • tagRFP-T (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The TagRFP-T in this plasmid contains S162T compared to TagRFP. It does not contain the C-terminal GMDELYK described in Shaner et al., 2008.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RHPMP190 (pLifeAct tagRFP-T) was a gift from Ricardo Henriques (Addgene plasmid # 88842 ; http://n2t.net/addgene:88842 ; RRID:Addgene_88842)
Related items:
Commonly requested with: