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Addgene

pSV Sport SREBP-1c
(Plasmid #8883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV-Sport
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3160
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SREBP-1c
  • Alt name
    ADD1
  • Alt name
    SREBP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    Active version of SREBP-1c.
  • Entrez Gene
    Srebf1 (a.k.a. ADD1, SREBP1, bHLHd1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that this is a mouse SREBP-1c, while the paper reference is a rat SREBP-1c. SREBP-1c differs from SREBP1a over amino acids 3-5 and contains a deletion of amino acids 6-29. See Figure 1c of the associated publication for more information.

Addgene's sequencing results identified a deletion of aa90-131 of SREBP1c compared to GenBank sequence NP_035610.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV Sport SREBP-1c was a gift from Bruce Spiegelman (Addgene plasmid # 8883 ; http://n2t.net/addgene:8883 ; RRID:Addgene_8883)

  • For your References section:

    ADD1: a novel helix-loop-helix transcription factor associated with adipocyte determination and differentiation. Tontonoz P, Kim JB, Graves RA, Spiegelman BM. Mol Cell Biol. 1993 Aug . 13(8):4753-9. PubMed 8336713
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