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Addgene

3702 pGEX4T-3 MLL AD F2852Y
(Plasmid #8874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T3
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLL min A.D.
  • Alt name
    MLL
  • Alt name
    minimal activation domain (aa 2829-2883)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    165
  • Mutation
    F2852Y: ILPSDIMDFVL --> ILPSDIMDYVL
  • Entrez Gene
    KMT2A (a.k.a. ALL-1, ALL1, CXXC7, GAS7, HRX, HTRX, HTRX1, MLL, MLL1, MLL1A, TRX1, WDSTS)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pGEX-5'
  • 3′ sequencing primer pGEX-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3702 pGEX4T-3 MLL AD F2852Y was a gift from Stanley Korsmeyer (Addgene plasmid # 8874 ; http://n2t.net/addgene:8874 ; RRID:Addgene_8874)

  • For your References section:

    MLL and CREB bind cooperatively to the nuclear coactivator CREB-binding protein. Ernst P, Wang J, Huang M, Goodman RH, Korsmeyer SJ. Mol Cell Biol. 2001 Apr . 21(7):2249-58. 10.1128/MCB.21.7.2249-2258.2001 PubMed 11259575
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