pPS934
(Plasmid
#8855)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8855 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS314
- Backbone size w/o insert (bp) 4800
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNUF2-sGFP
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Alt namepJK52
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2500
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Entrez GeneNUF2
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CEN plasmid. NUF2 promoter, NUF2 ORF, sGFP, NUF2 3' UTR. See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS934 was a gift from Pamela Silver (Addgene plasmid # 8855 ; http://n2t.net/addgene:8855 ; RRID:Addgene_8855) -
For your References section:
The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Kahana JA, Schlenstedt G, Evanchuk DM, Geiser JR, Hoyt MA, Silver PA. Mol Biol Cell. 1998 Jul . 9(7):1741-56. 10.1091/mbc.9.7.1741 PubMed 9658168
Map uploaded by the depositor.
