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pPS1622
(Plasmid #8854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4895
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Sec63-sGFP
  • Alt name
    Sec63
  • Alt name
    pJK59
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    sGFP=S65T V163A mutant of GFP
  • Entrez Gene
    SEC63 (a.k.a. YOR254C, PTL1)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by amplifying SEC63 and ~400 bp of its 5' promoter from pPS599 (R. Schekman, UC Berkeley). Product cloned between XbaI and XhoI sites of CEN/URA3 plasmid, pJK50 (Kahana, MBC 1998).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1622 was a gift from Pamela Silver (Addgene plasmid # 8854 ; http://n2t.net/addgene:8854 ; RRID:Addgene_8854)

  • For your References section:

    Mutants affecting the structure of the cortical endoplasmic reticulum in Saccharomyces cerevisiae. Prinz WA, Grzyb L, Veenhuis M, Kahana JA, Silver PA, Rapoport TA. J Cell Biol. 2000 Aug 7. 150(3):461-74. 10.1083/jcb.150.3.461 PubMed 10931860
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