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Addgene

pPS1701
(Plasmid #8852)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NIP100
  • Alt name
    pJK138
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    NIP100 (a.k.a. YPL174C, PAC13)
  • Tag / Fusion Protein
    • 3x HA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Integrating Nip100, 3xHA, NUF2 3'UTR plasmid. Rescues nip100delta; cin8delta. Cut with ClaI before transforming. Note that XhoI fusion site was destroyed because SalI was used to clone Nip100 (which has an internal XhoI site).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1701 was a gift from Pamela Silver (Addgene plasmid # 8852 ; http://n2t.net/addgene:8852 ; RRID:Addgene_8852)

  • For your References section:

    The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Kahana JA, Schlenstedt G, Evanchuk DM, Geiser JR, Hoyt MA, Silver PA. Mol Biol Cell. 1998 Jul . 9(7):1741-56. 10.1091/mbc.9.7.1741 PubMed 9658168
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