pPS904
(Plasmid
#8850)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonena
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNUF2-sGFP
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneNUF2
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
2 micron plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS904 was a gift from Pamela Silver (Addgene plasmid # 8850 ; http://n2t.net/addgene:8850 ; RRID:Addgene_8850) -
For your References section:
Kinetics of spindle pole body separation in budding yeast. Kahana JA, Schnapp BJ, Silver PA. Proc Natl Acad Sci U S A. 1995 Oct 10. 92(21):9707-11. 10.1073/pnas.92.21.9707 PubMed 7568202