pDblet
(Plasmid #8848)

• Depositing Lab: Joel Huberman
• Publication: Brun et al Gene. 1995 Oct 16. 164(1):173-7.

Backbone

• Vector backbone: pBluescribe
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5085
• Vector type: Yeast Expression ; yeast cloning vector
• Selectable markers: URA3 ; ura4 (pombe gene equivalent to )

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ARS doublet
• Species: S. pombe (fission yeast)
• Insert Size (bp): 1198
• Mutation: This vector contains, between AatII sites, a direct repeat of nucleotides 3289-3870 from GenBank sequence Z27236, which corresponds to the sequence published in Zhu, Carlson, Dubey, Sharma and Huberman, Chromosoma 103: 414-422, 1994. This corresponds to a portion of S. pombe's ars3002, a functional chromosomal DNA replication origin. The duplication dramatically increases the replication efficiency of the origin.
• GenBank ID: AF531173

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AatII (not destroyed)
• 3′ cloning site: AatII (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Thanks to its ARS doublet sequence, this plasmid very efficiently transforms S. pombe cells, and it replicates autonomously in the nuclei of such cells. Although it has a pBluescribe backbone, pDblet contains the SK+ multiple cloning site from pBluescript II.