pDW465
(Plasmid #8846)

• Purpose: (Empty Backbone)
• Depositing Lab: David Waugh
• Publication: Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Backbone

• Vector backbone: p2Bac
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 9500
• Vector type: Insect Expression
• Tag / Fusion Protein:
  • biotin acceptor peptide (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pDW464/465 Cloning sites

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This baculovirus transfer vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused to its C-terminus in Spodoptera frugiperda cells. pDW465 also overproduces E. coli biotin holoenzyme synthetase (BirA), under polyhedron promoter control, so that BAP-tagged proteins can be biotinylated in Spodoptera frugiperda cells. pDW465 is designed to use in conjunction with the Bac-to-Bac system (Invitrogen) for constructing recombinant baculoviruses.