pDW446
(Plasmid
#8844)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep2Bac
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Backbone manufacturerInvitrogen
- Backbone size (bp) 9500
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Vector typeInsect Expression
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Tag
/ Fusion Protein
- biotin acceptor peptide (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This baculovirus transfer vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused to its C-terminus in Spodoptera frugiperda cells. Also produces BirA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDW446 was a gift from David Waugh (Addgene plasmid # 8844 ; http://n2t.net/addgene:8844 ; RRID:Addgene_8844) -
For your References section:
Site-specific, enzymatic biotinylation of recombinant proteins in Spodoptera frugiperda cells using biotin acceptor peptides. Duffy S, Tsao KL, Waugh DS. Anal Biochem. 1998 Sep 10. 262(2):122-8. 10.1006/abio.1998.2770 PubMed 9750126
