pDW363
(Plasmid #8842)

• Purpose: (Empty Backbone)
• Depositing Lab: David Waugh
• Publication: Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Backbone

• Vector backbone: pMal-C2
• Backbone manufacturer: New England Biolabs
• Backbone size (bp): 6700
• Modifications to backbone: Natural N-terminal signal peptide of MBP deleted and replaced by biotin acceptor peptide.
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • biotin acceptor peptide (N terminal on backbone)
  • MBP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MBP-F
• 3′ sequencing primer: M13_pUC_fwd; M13-F20

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid expression vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused directly to its N-terminus, or, alternatively, as a fusion to the C-terminus of E. coli maltose-binding protein with the BAP on its N-terminus. pDW363 also overproduces biotin holoenzyme synthetase (BirA) to ensure efficient biotinylation of BAP-tagged proteins in vivo. Add biotin to the medium (50 mM) when cultivating cells that contain pDW363 or it derivatives.