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Addgene

pKM999
(Plasmid #8839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8839 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET11
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 5700
  • Modifications to backbone
    DNA encoding presumptive N-terminal signal peptide deleted.
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • T. litoralis MBP (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Because it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
  • Copy number
    Low Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of T. litoralis maltose-binding protein. Presumptive N-terminal signal peptide deleted.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM999 was a gift from David Waugh (Addgene plasmid # 8839)

  • For your References section:

    Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Fox JD, Routzahn KM, Bucher MH, Waugh DS. FEBS Lett. 2003 Feb 27. 537(1-3):53-7. 10.1016/S0014-5793(03)00070-X PubMed 12606030
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