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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepZA31
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Backbone manufacturerHerman Bujard Lab
- Backbone size w/o insert (bp) 6700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BL21-Pro
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Growth instructionsFor best results, perform intracellular processing experiments at 30C.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTVMV protease, catalytic domain
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Alt nametobacco vein mottling virus protease
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Speciestobacco vein mottling virus
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Insert Size (bp)700
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MfeI (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer pLTet-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK1037 was a gift from David Waugh (Addgene plasmid # 8833 ; http://n2t.net/addgene:8833 ; RRID:Addgene_8833) -
For your References section:
Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Nallamsetty S, Kapust RB, Tozser J, Cherry S, Tropea JE, Copeland TD, Waugh DS. Protein Expr Purif. 2004 Nov . 38(1):108-15. 10.1016/j.pep.2004.08.016 PubMed 15477088
