pRK603
(Plasmid #8831)

• Depositing Lab: David Waugh
• Publication: Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312-8.

Backbone

• Vector backbone: pZA31
• Backbone manufacturer: Herman Bujard Lab
• Backbone size w/o insert (bp): 6700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21-Pro
• Growth instructions: For best results, perform intracellular processing experiments at 30C.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TEV protease, S219D mutant
• Alt name: tobacco etch virus protease
• Species: tobacco etch virus
• Insert Size (bp): 700
• Mutation: S219D mutant

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pLTet-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ATCC
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. Expression of the TEV protease gene on pRK603 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech).