pDONR223-ZNF232
(Plasmid
#88305)
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PurposeDonor Vector containing ZNF232 transcription factor, part of the Human TFome Collection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 88305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR223
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3000
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Vector typeGateway Donor Vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZNF232
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SpeciesH. sapiens (human)
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Insert Size (bp)1254
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MutationLast nucleotide of stop codon removed to allow for Gateway C-terminal fusions
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Entrez GeneZNF232 (a.k.a. ZSCAN11)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F(-21)
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Represents the ORFeome subset of the TFome
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223-ZNF232 was a gift from Marc Vidal (Addgene plasmid # 88305 ; http://n2t.net/addgene:88305 ; RRID:Addgene_88305) -
For your References section:
A comprehensive library of human transcription factors for cell fate engineering. Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal M, Melero-Martin JM, Busskamp V, Church GM. Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 10.1038/s41587-020-0742-6. Epub 2020 Nov 30. 10.1038/s41587-020-0742-6 PubMed 33257861
