pET26b-Granzyme A (S-A)
(Plasmid #8825)

• Depositing Lab: Judy Lieberman
• Publication: Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(17):9285-90.

Backbone

• Vector backbone: pET26b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5360
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For protein expression, use only BL21(DE3) competent cell from Novagen
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human Granzyme A inactive
• Alt name: Granzyme A (S>A)
• Alt name: GZMA
• Species: H. sapiens (human)
• Insert Size (bp): 704
• Mutation: changed Serine 184 to Alanine
• GenBank ID: NP_006135
• Entrez Gene: GZMA (a.k.a. CTLA3, HFSP)
• Tag / Fusion Protein:
  • (His)6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry