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pET30a-PP32
(Plasmid #8824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8824 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET30a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5422
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    for protein expression, use only BL21(DE3) competent cell from Novagen
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP32
  • Alt name
    PHAP I
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    747
  • Mutation
    mutation E246Q and deletion of E209.
  • GenBank ID
    NM_006305
  • Entrez Gene
    ANP32A (a.k.a. C15orf1, HPPCn, I1PP2A, LANP, MAPM, PHAP1, PHAPI, PP32)
  • Tags / Fusion Proteins
    • 6XHIS (N terminal on backbone)
    • S-Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a-PP32 was a gift from Judy Lieberman (Addgene plasmid # 8824)

  • For your References section:

    Granzyme A activates an endoplasmic reticulum-associated caspase-independent nuclease to induce single-stranded DNA nicks. Beresford PJ, Zhang D, Oh DY, Fan Z, Greer EL, Russo ML, Jaju M, Lieberman J. J Biol Chem 2001 Nov 16;276(46):43285-93. Epub 2001 Sep 12. 10.1074/jbc.M108137200 PubMed 11555662
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