pUB23-K-beta-gal
(Plasmid
#8819)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUB23
- Backbone size w/o insert (bp) 10500
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-gal
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SpeciesE. coli
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MutationK (lysine) at amino terminus of beta-gal
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Tag
/ Fusion Protein
- Ubiquitin (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer lacZ-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Half life of beta-gal ~3 minutes. See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUB23-K-beta-gal was a gift from Alexander Varshavsky (Addgene plasmid # 8819 ; http://n2t.net/addgene:8819 ; RRID:Addgene_8819) -
For your References section:
In vivo half-life of a protein is a function of its amino-terminal residue. Bachmair A, Finley D, Varshavsky A. Science 1986 Oct 10;234(4773):179-86. 10.1126/science.3018930 PubMed 3018930
Map uploaded by the depositor.
