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Addgene

PBB-79
(Plasmid #8813)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8813 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pG5'
  • Backbone size w/o insert (bp) 3350
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GABPb1 (1-157)
  • Alt name
    GABPb1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    471
  • Entrez Gene
    Gabpb1 (a.k.a. E4TF1, GABPB, BABPB2, E4Tf1B, NRF2B1, NRF2B2, E4TF1-47, E4TF1-53, GABPB1-1, GABPB1-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PBB-79 was a gift from Cynthia Wolberger (Addgene plasmid # 8813 ; http://n2t.net/addgene:8813 ; RRID:Addgene_8813)

  • For your References section:

    The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Batchelor AH, Piper DE, de la Brousse FC, McKnight SL, Wolberger C. Science 1998 Feb 13;279(5353):1037-41. 10.1126/science.279.5353.1037 PubMed 9461436
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