3122 pSFFV-neo Bcl-XL MI-3
(Plasmid
#8811)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSFFV-neo
- Backbone size w/o insert (bp) 7200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcl-XL MI-3
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Alt nameBcl-XL
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SpeciesH. sapiens (human)
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Insert Size (bp)700
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MutationBH1 WGR-->WAR
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GenBank IDNM_138578
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Entrez GeneBCL2L1 (a.k.a. BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SFFV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3122 pSFFV-neo Bcl-XL MI-3 was a gift from Stanley Korsmeyer (Addgene plasmid # 8811 ; http://n2t.net/addgene:8811 ; RRID:Addgene_8811) -
For your References section:
Bcl-XL and Bcl-2 repress a common pathway of cell death. Chao DT, Linette GP, Boise LH, White LS, Thompson CB, Korsmeyer SJ. J Exp Med 1995 Sep 1;182(3):821-8. 10.1084/jem.182.3.821 PubMed 7650488