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Addgene

pDONR223-ZNF22
(Plasmid #88102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 88102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Gateway Donor Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF22
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    675
  • Mutation
    Last nucleotide of stop codon removed to allow for Gateway C-terminal fusions
  • Entrez Gene
    ZNF22 (a.k.a. KOX15, HKR-T1, ZNF422, Zfp422)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Represents the ORFeome subset of the TFome

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223-ZNF22 was a gift from Marc Vidal (Addgene plasmid # 88102 ; http://n2t.net/addgene:88102 ; RRID:Addgene_88102)

  • For your References section:

    A comprehensive library of human transcription factors for cell fate engineering. Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal M, Melero-Martin JM, Busskamp V, Church GM. Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 10.1038/s41587-020-0742-6. Epub 2020 Nov 30. 10.1038/s41587-020-0742-6 PubMed 33257861
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