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Addgene

3694 pMIG HA-Bax
(Plasmid #8806)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8806 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIG
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bax
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    580
  • GenBank ID
    NM_007527
  • Entrez Gene
    Bax
  • Tags / Fusion Proteins
    • GFP (C terminal on backbone)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Bgl II (unknown if destroyed)
  • 5′ sequencing primer pLXSN 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3694 pMIG HA-Bax was a gift from Stanley Korsmeyer (Addgene plasmid # 8806 ; http://n2t.net/addgene:8806 ; RRID:Addgene_8806)

  • For your References section:

    VDAC2 inhibits BAK activation and mitochondrial apoptosis. Cheng EH, Sheiko TV, Fisher JK, Craigen WJ, Korsmeyer SJ. Science 2003 Jul 25;301(5632):513-7. 10.1126/science.1083995 PubMed 12881569
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