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Addgene

3605 pGEX4T-1 Bad S112A S136A (104-141)
(Plasmid #8803)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bad
  • Alt name
    BCL2-associated agonist of cell death
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    100
  • Mutation
    Contains AA's 104-141; S112A, S136A
  • GenBank ID
    NM_007522 NP_031548.1
  • Entrez Gene
    Bad (a.k.a. AI325008, Bbc2)
  • Promoter Ptac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3605 pGEX4T-1 Bad S112A S136A (104-141) was a gift from Stanley Korsmeyer (Addgene plasmid # 8803 ; http://n2t.net/addgene:8803 ; RRID:Addgene_8803)

  • For your References section:

    p70S6 kinase signals cell survival as well as growth, inactivating the pro-apoptotic molecule BAD. Harada H, Andersen JS, Mann M, Terada N, Korsmeyer SJ. Proc Natl Acad Sci U S A 2001 Aug 14;98(17):9666-70. Epub 2001 Aug 7. 10.1073/pnas.171301998 PubMed 11493700
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