3604 pGEX4T-1 Bad S136A (104-141)
(Plasmid #8801)

• Depositing Lab: Stanley Korsmeyer
• Publication: Harada et al Mol Cell 1999 Apr;3(4):413-22.

Backbone

• Vector backbone: pGEX-4T1
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bad
• Alt name: BCL2-associated agonist of cell death
• Species: M. musculus (mouse)
• Insert Size (bp): 100
• Mutation: Contains AA's 104-141; S136A
• GenBank ID: NM_007522 NP_031548.1
• Entrez Gene: Bad (a.k.a. AI325008, Bbc2)
• Promoter: Ptac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry