pLJC2-Rap2A-3xFLAG
(Plasmid #87974)

• Purpose: 3rd generation lentiviral vector for Expression of Rap2A in mammalian cells
• Depositing Lab: David Sabatini
• Publication: Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi: 10.1016/j.cell.2017.03.023.

Backbone

• Vector backbone: pLJC2
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RAP2A
• Species: H. sapiens (human)
• Entrez Gene: RAP2A (a.k.a. K-REV, KREV, RAP2, RbBP-30)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: TGTACGGTGGGAGGTCTATATAAG
• 3′ sequencing primer: CCCTTTTCTTTTAAAATTGTGGATGAATACTGCC

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry