pms5056(JBEI-10534)
(Plasmid
#87963)
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PurposejStack yeast assembly into plant vectors
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87963 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBca9145
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name{T_tNos}
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Alt nameNopaline Synthase terminator
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pms5056(JBEI-10534) was a gift from Dominique Loque (Addgene plasmid # 87963 ; http://n2t.net/addgene:87963 ; RRID:Addgene_87963) -
For your References section:
A robust gene-stacking method utilizing yeast assembly for plant synthetic biology. Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, Loque D. Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215. 10.1038/ncomms13215 PubMed 27782150