pEGFPN1-gamma-tubulin
(Plasmid #87956)

• Purpose: Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein
• Depositing Labs: Maria Alvarado-Kristensson, Ana Carrera
• Publication: Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 2009 Aug 2.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gamma-tubulin 1
• Alt name: TUBG1
• Species: H. sapiens (human)
• Insert Size (bp): 345
• Mutation: The protein is a sh-gamma-tubulin resistant gene (mutation R47H) only AA1-340 of TUBG1 are present.
• GenBank ID: NM_001070.4
• Entrez Gene: TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TUBG1 was originally cloned by Dr. Jiri Bartek, I subclonned the protein into the pEGFN1 vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The protein is a sh-gamma-tubulin resistant gene.