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pTER GAMMA-TUBULIN shRNA Human
(Plasmid #87955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTER
  • Backbone manufacturer
    A gift from Dr. Marc van de Wetering
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sh gamma-tubulin
  • gRNA/shRNA sequence
    TUBG1 and TUBG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    345
  • Mutation
    The protein is a sh-gamma-tubulin resistant gene. C 1236 and 1242 are mutated to T
  • GenBank ID
    NM_001070.4
  • Entrez Gene
    TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
  • Tag / Fusion Protein
    • No-tag

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTER GAMMA-TUBULIN shRNA Human was a gift from Maria Alvarado-Kristensson & Ana Carrera (Addgene plasmid # 87955 ; http://n2t.net/addgene:87955 ; RRID:Addgene_87955)

  • For your References section:

    SADB phosphorylation of gamma-tubulin regulates centrosome duplication. Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera AC. Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 2009 Aug 2. 10.1038/ncb1921 PubMed 19648910
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