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Addgene

3576 pSFFV-neo Bad R149A
(Plasmid #8795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV-neo
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bad
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    615
  • Mutation
    R149A
  • GenBank ID
    NM_007522
  • Entrez Gene
    Bad (a.k.a. AI325008, Bbc2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SFFV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3576 pSFFV-neo Bad R149A was a gift from Stanley Korsmeyer (Addgene plasmid # 8795 ; http://n2t.net/addgene:8795 ; RRID:Addgene_8795)

  • For your References section:

    BH3 domain of BAD is required for heterodimerization with BCL-XL and pro-apoptotic activity. Zha J, Harada H, Osipov K, Jockel J, Waksman G, Korsmeyer SJ. J Biol Chem 1997 Sep 26;272(39):24101-4. 10.1074/jbc.272.39.24101 PubMed 9305851
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