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pDRf1-4CL5-AtSCT (JBEI-11557)
(Plasmid #87937)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDRf1
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    AtSCT
  • Alt name
    p-coumaroyl-CoA:spermidine
  • Species
    A. thaliana (mustard weed)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    4CL5
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    4CL5 (a.k.a. AT3G21230, 4-coumarate:CoA ligase 5)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRf1-4CL5-AtSCT (JBEI-11557) was a gift from Dominique Loque (Addgene plasmid # 87937 ; http://n2t.net/addgene:87937 ; RRID:Addgene_87937)

  • For your References section:

    Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast. Eudes A, Mouille M, Robinson DS, Benites VT, Wang G, Roux L, Tsai YL, Baidoo EE, Chiu TY, Heazlewood JL, Scheller HV, Mukhopadhyay A, Keasling JD, Deutsch S, Loque D. Microb Cell Fact. 2016 Nov 21;15(1):198. 10.1186/s12934-016-0593-5 [pii] PubMed 27871334
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