pDRf1-4CL5-AtSDT (JBEI-11535)
(Plasmid
#87934)
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PurposeCo-expression in S.cerevisiae of 4CL5 and AtSDT
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDRf1
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameSDT
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SpeciesA. thaliana (mustard weed)
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Entrez GeneSDT (a.k.a. AT2G23510, F26B6.16, F26B6_16, spermidine disinapoyl acyltransferase)
Cloning Information for Gene/Insert 1
- Cloning method Gateway Cloning
- 5′ sequencing primer pPMA-For (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert name4CL5
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SpeciesA. thaliana (mustard weed)
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Entrez Gene4CL5 (a.k.a. AT3G21230, 4-coumarate:CoA ligase 5)
Cloning Information for Gene/Insert 2
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDRf1-4CL5-AtSDT (JBEI-11535) was a gift from Dominique Loque (Addgene plasmid # 87934 ; http://n2t.net/addgene:87934 ; RRID:Addgene_87934) -
For your References section:
Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast. Eudes A, Mouille M, Robinson DS, Benites VT, Wang G, Roux L, Tsai YL, Baidoo EE, Chiu TY, Heazlewood JL, Scheller HV, Mukhopadhyay A, Keasling JD, Deutsch S, Loque D. Microb Cell Fact. 2016 Nov 21;15(1):198. 10.1186/s12934-016-0593-5 [pii] PubMed 27871334