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pRK5-ATP6V1D-HAGST
(Plasmid #87932)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4061
  • Total vector size (bp) 5632
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATP6V1D
  • Alt name
    VATD; VMA8; ATP6M
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    740
  • GenBank ID
    NM_015994.3
  • Entrez Gene
    ATP6V1D (a.k.a. ATP6M, VATD, VMA8)
  • Tag / Fusion Protein
    • HA-GST (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer SP6 : ATT TAG GTG ACA CTA TAG
  • 3′ sequencing primer pRK5-3': TGT AAC CAT TAT AAG CTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human cDNA for ATP6V1D was purchased from Open Biosystems

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-ATP6V1D-HAGST was a gift from David Sabatini (Addgene plasmid # 87932 ; http://n2t.net/addgene:87932 ; RRID:Addgene_87932)

  • For your References section:

    mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase. Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM. Science. 2011 Nov 4;334(6056):678-83. 10.1126/science.1207056 PubMed 22053050
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