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pET21a_Msm_RbpA
(Plasmid #87928)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 5788
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression
  • Selectable markers
    ampicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To express use BL21 (DE3) strains
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RbpA
  • Species
    Mycobacterium smegmatis
  • Insert Size (bp)
    345
  • GenBank ID
    HQ203032.1
  • Promoter T7P

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer t7p
  • 3′ sequencing primer t7t
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a_Msm_RbpA was a gift from Seth Darst (Addgene plasmid # 87928 ; http://n2t.net/addgene:87928 ; RRID:Addgene_87928)

  • For your References section:

    Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA. Hubin EA, Fay A, Xu C, Bean JM, Saecker RM, Glickman MS, Darst SA, Campbell EA. Elife. 2017 Jan 9;6. pii: e22520. doi: 10.7554/eLife.22520. 10.7554/eLife.22520 PubMed 28067618
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