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pcDNA3.1-IDH2-R172K
(Plasmid #87927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen, cat# V790-20
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial
  • Alt name
    IDH2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1359
  • Mutation
    R172K
  • GenBank ID
    NM_002168.3
  • Entrez Gene
    IDH2 (a.k.a. D2HGA2, ICD-M, IDH, IDH-2, IDHM, IDP, IDPM, mNADP-IDH)
  • Promoter CMV
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IDH2 R172K was amplified by PCR from pCMV6-Entry vectors (Origene, cat# RC400103

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-IDH2-R172K was a gift from Reproducibility Project Cancer Biology (Addgene plasmid # 87927 ; http://n2t.net/addgene:87927 ; RRID:Addgene_87927)

  • For your References section:

    Replication Study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate. Showalter MR, Hatakeyama J, Cajka T, VanderVorst K, Carraway KL, Fiehn O. Elife. 2017 Jun 27;6. pii: e26030. doi: 10.7554/eLife.26030. 10.7554/eLife.26030 PubMed 28653623
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